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Image Search Results
Journal: Annals of Translational Medicine
Article Title: Delay in articular cartilage degeneration of the knee joint by the conditional removal of discoidin domain receptor 2 in a spontaneous mouse model of osteoarthritis
doi: 10.21037/atm-20-5786
Figure Lengend Snippet: Immunohistostaining of Ddr2, MMP-13, and degraded collagen type II in the articular cartilage of mouse knee joints at 9 months of age. The protein expression of Ddr2, MMP-13, and degraded collagen type II were increased in oil-treated Acan+/CreERT2;Ddr2flox/flox;Col11a1+/− mice (as indicated by the brown-color–stained cells in the middle column of the figure). However, the protein expression of Ddr2, MMP-13, and degraded collagen type II were not detected in the articular cartilage of tamoxifen-treated Acan+/CreERT2;Ddr2flox/flox; Col11a1+/− mice (Acan+/CreERT2;Ddr2Δ/Δ;Col11a1+/−; right column) and wild-type mice (left column). The dashed line represents the boundary between articular cartilage (AC) and subchondral bone (SB). Bar =100 µm.
Article Snippet: The sections were incubated with the antibody against Ddr2 (1:300 dilution, Abcam, cat. no. ab203219), the antibody against MMP-13 (1:300 dilution, Abcam, cat. no. ab84594), or the
Techniques: Expressing, Staining